What is the purpose of guaiacol in the activity of peroxidase?
What is the purpose of guaiacol in the activity of peroxidase?
Guaiacol peroxidases are heme-containing proteins that preferably oxidize aromatic electron donors such as guaiacol and pyragallol at the expense of H2O2 (Sharma et al. 2012). Guaiacol peroxidase is associated with many important biosynthetic processes and defense against abiotic and biotic stresses.
What is peroxidase assay?
Peroxidase Assay Kit uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (λexc = 530nm, λem = 590nm) is a direct measure of the enzyme activity.
What does guaiacol mean?
Medical Definition of guaiacol : a fragrant liquid or solid compound C7H8O2 obtained by distilling guaiacum or from wood-tar creosote or made synthetically and used chiefly as an expectorant and as a local anesthetic.
What is the role of guaiacol in the reaction catalyzed by turnip peroxidase?
In the reaction, guaiacol will donate hydrogens and thereby become oxidized. It has a brown color in its oxidized form; you will be able to measure the amount of oxidized guaiacol produced by determining the intensity of the color in the spectrophotometer.
What is hemoglobin peroxidase?
As a hemoprotein, hemoglobin (Hb) can, in the presence of H2O2, act as a peroxidase. In red blood cells, this activity is regulated by the reducing environment.
How can we tell if catalase is active?
The catalase test tests for the presence of catalase, an enzyme that breaks down the harmful substance hydrogen peroxide into water and oxygen. If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it.
Where is peroxidase found in the human body?
Thyroid peroxidase also called as thyroperoxidase (TPO) is mainly expressed in thyroid organs. It is a large transmembrane glycoprotein with covalently linked haem, present in cells on the apical membrane (Gardas et al., 1999).
What is the optimal pH for peroxidase?
The optimal pH of the purified peroxidase was 5.0 and its activity was retained at pH values between 5.0–10.0. The enzyme was heat stable over a wide range of temperatures (0–60°C), and less than 50% of its activity was lost at 70°C after incubation for 30 min.
What does guaiacol smell like?
Guaiacol and its derivatives are commonly found in nature and are known for their characteristic smoky, clove-like and vanilla-like smells.