What is linear polyacrylamide?

What is linear polyacrylamide?

Linear polyacrylamide is a very efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. Polyacrylamide has several advantages over other available carriers for working with DNA or studying DNA-protein interactions.

How do you make linear acrylamide?

Preparation of Linear Acrylamide

1. Dissolve 5mg Acrylamide in 200 µl TE.
2. Add 1 µl 10%APS.
3. Add 1 µl TEMED.
4. Allow to polymerize (incubate as long as you like, can heat to 37°C to increase rate): forms a jelly mass.
5. Add 2.5 volumes ethanol.
6. Spin 5-6 minutes.
7. Speed vac to dry.
8. Resuspend 500 µl ddH20 (i.e. 10 µg/µl).

Where is acrylamide found?

Acrylamide is found mainly in foods made from plants, such as potato products, grain products, or coffee. Acrylamide does not form, or forms at lower levels, in dairy, meat, and fish products. Generally, acrylamide is more likely to accumulate when cooking is done for longer periods or at higher temperatures.

How do you prepare glycogen solution?

Start by dissolving 5 gr. of glycogen (from Oyster, Sigma G 8751) in 10 ml of milli-Q water. Extract once with one volume of Phenol followed by one extraction with one volume of Chlorophorm. Add one volume of absolute ethanol to the supernatant, at this point the glycogen will precipitate instantaneously.

How is polyacrylamide made?

Polyacrylamide gels are prepared by free radical polymerization of acrylamide and a comonomer crosslinker such as bis-acrylamide. Polymerization is initiated by ammonium persulfate (APS) with tetramethylethylenediamine (TEMED) as the catalyst (see figure below).

Why is glycogen insoluble in ethanol?

Concentration: 20 mg/ml aqueous solution. Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier, free of host DNA/RNA. Glycogen is insoluble in ethanol solution; in the presence of salts it forms a precipitate that traps the target nucleic acids.

Does glycogen dissolve in water?

Glycogen is a white amorphous powder, poorly soluble in water, and readily hydrolyzed by mineral acids to yield glucose residues.

Why polyacrylamide is soluble in water?

Poly(acrylamide) is infinitely soluble in water. In a way this is counterintuitive because we are accustomed to thinking of a polymer solution as consisting of a polymer dissolved in some small molecule substance….Properties of Acrylamide Monomer.

Why does glycogen precipitate in alcohol?

It is an inert carrier, free of host DNA/RNA. Glycogen is insoluble in ethanol solution; in the presence of salts it forms a precipitate that traps the target nucleic acids.