What is CDC6 and CDT1?
What is CDC6 and CDT1?
CDC6 is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). CDC6 assembles after ORC in an ATP dependent manner and is required for loading MCM proteins onto the DNA.
What does CDT1 stand for?
Chromatin licensing and DNA replication factor 1
CDT1 (Chromatin licensing and DNA replication factor 1) is a protein that in humans is encoded by the CDT1 gene. It is a licensing factor that functions to limit DNA from replicating more than once per cell cycle. CDT1.
What is the function of CDT1?
Normal Function The CDT1 gene provides instructions for making a protein that is important in the copying of a cell’s DNA before the cell divides (a process known as DNA replication). The protein produced from this gene is one of a group of proteins known as the pre-replication complex.
What is DDK protein?
DDK phosphorylates the meiosis-specific double-strand break protein MER2 for initiation of meiotic recombination. Interacts with CDC5 during meiosis to promote double-strand breaks and monopolar spindle orientation. Inhibits CDC5 activity during mitosis through direct binding to its PBD.
Is CDC6 degraded?
Cdc6 is an unstable protein that is rapidly degraded at the G1 to S phase transition (Piatti et al., 1996; Drury et al., 1997; Sánchez et al., 1999) and that must be synthesized de novo in each cell cycle.
Is CDC6 a cyclin?
The cyclin binding domain of CDC6 was mapped to an N-terminal Cy-motif that is similar to the cyclin binding regions in p21(WAF1/SDI1) and E2F-1.
What genetic diseases are associated with CDT1?
CDT1 (Chromatin Licensing And DNA Replication Factor 1) is a Protein Coding gene. Diseases associated with CDT1 include Meier-Gorlin Syndrome 4 and Meier-Gorlin Syndrome 1. Among its related pathways are Activation of the pre-replicative complex and Regulation of activated PAK-2p34 by proteasome mediated degradation.
How is CDT1 activated?
In vitro, Cdt1 can be phosphorylated by Cdk4 (activated in G1 by cyclin D), Cdk2 (activated in S by cyclin E and cyclin A) and Cdk1 (activated by cyclin A and cyclin B) [124,125]. Moreover, Cdt1 isolated from cell lysates co-precipitates cyclin A, but not cyclin E or cyclin B.
How is DDK calculated?
The DDK rate is calculated by dividing the total number of iterations by the duration of the trial or by determining the time it took the client to make a set number of iterations. The results are scored and compared to the published normative values.
What is CDK and DDK?
They require the activities of two types of serine-threonine kinases, the cyclin-dependent kinases (CDKs) and the Dbf4-dependent kinase (DDK). CDK and DDK are essential for genome duplication and maintenance in both mitotic and meiotic divisions.
What happens if Cdc6 Cannot be phosphorylated?
You create cells with a version of Cdc6 that cannot be phosphorylated and thus cannot be degraded.
What is the function of Cdc6 during S phase?
Cdc6 is required for formation of pre-replication complexes during DNA replication. Phosphorylation of Cdc6 by S-phase cyclin-dependent kinases is the rate-limiting step for initiation of DNA replication.