What is alkaline phosphatase isoenzymes testing for?

What is alkaline phosphatase isoenzymes testing for?

When the ALP test result is high, you may need to have the ALP isoenzyme test. This test will help determine what part of the body is causing higher ALP levels. This test may be used to diagnose or monitor: Bone disease. Liver, gallbladder, or bile duct disease.

What is fractionated alkaline phosphatase test?

This test will help determine what part of the body is causing higher ALP levels. This test may be used to diagnose or monitor: Bone disease. Liver , gallbladder, or bile duct disease.

What are the techniques used in the separation of ALP isoenzymes?

Serum Enzymes The same electrophoretic techniques are used for the separation of ALP isoenzymes in serum as for separation of serum proteins.

What are various isoenzymes of alkaline phosphatase?

Alkaline phosphatase is divided into four isozymes depending upon the site of tissue expression that are Intestinal ALP, Placental ALP, Germ cell ALP and tissue nonspecific alkaline phosphatase or liver/bone/kidney (L/B/K) ALP.

What causes elevated liver isoenzymes?

Liver diseases, medical conditions, medications and infections can cause elevated liver enzymes. Common causes for elevated liver enzymes include: Certain medications, such as cholesterol-lowering drugs (statins) and acetaminophen. Fatty liver disease, both alcoholic and nonalcoholic.

What is high bone isoenzymes?

Higher-than-normal levels of ALP bone isoenzyme could indicate a bone disease such as: osteoblastic bone tumors. osteomalacia, or rickets. osteoporosis. Paget’s disease of bone.

What is alkaline phosphatase Slideshare?

INTRODUCTION. TOPIC. LOCATION • ALP present in all tissues but it is particularly concentrated in liver, bile duct,kidney,bones,placenta,intestinal mucosa. CLINICAL SIGNIFICANCE OF ALP •ALP test is used to detect Liver disease,Bone disorders,biliary obstruction and cancer.

What is the significance of alkaline phosphatase?

An alkaline phosphatase (ALP) test measures the amount of ALP in your blood. Although ALP exists throughout your body, the two main sources of ALP in your blood are your liver and bones. High levels of ALP may indicate liver disease or certain bone disorders, but an ALP test alone cannot diagnose a condition.

What do you mean by isoenzyme?

Isoenzymes (also called isozymes) are alternative forms of the same enzyme activity that exist in different proportions in different tissues. Isoenzymes differ in amino acid composition and sequence and multimeric quaternary structure; mostly, but not always, they have similar (conserved) structures.

What does high alkaline phosphatase indicate?

ALP is an enzyme found throughout the body, but it is mostly found in the liver, bones, kidneys, and digestive system. When the liver is damaged, ALP may leak into the bloodstream. High levels of ALP can indicate liver disease or bone disorders.