What are the different types of hybridization techniques?
What are the different types of hybridization techniques?
Types of Hybridization:
- Hybridization may be of following types:
- (i) Intra-varietal hybridization:
- (ii) Inter-varietal or Intraspecific hybridization:
- (iv) Interspecific hybridization or intragenric hybridization:
- (v) Introgressive hybridization:
- It involves the following steps:
- (i) Selection of parents:
Why is nucleic acid hybridization important?
Nucleic acid hybridisation in quantitative analysis permits detection of mutations such as deletion, insertion and copy number variation for disease diagnosis.
Which techniques use DNA hybridisation?
DNA replication and transcription of DNA into RNA both rely upon nucleotide hybridization, as do molecular biology techniques including Southern blots and Northern blots, the polymerase chain reaction (PCR), and most approaches to DNA sequencing.
What is nucleic acid hybridization PDF?
The nucleic acid hybridization is the process wherein two DNA or RNA single chains (mono-stranded) from different biological sources, make the double catenary configuration, based on contingent sequence homology of the two sources, resulting DNA-DNA, RNA-RNA or DNA-RNA hybrids.
Who discovered nucleic acid hybridization?
The technique of nucleic acids hybridization was launched in 1956 by Alexander Rich (Box 9.7) and David Davies (1927–), both then at the NIH.
What is the objective of hybridization?
The chief objective of hybridization is to create variation. When two genotypically different plants are crossed, the genes from both the parents are brought together in Fl. Segregation and recombination produce many new gene combinations in F2 and subsequent generations.
When was nucleic acid hybridization discovered?
What factors affect hybridization?
Factors Affecting Hybridization
- Temperature: If the temperature is too high, the strands melt.
- The pH: A pH that is too alkaline will cause the strands to separate; too acidic and they are forced together.
What are the steps involved in the process of hybridization?
The major steps involved in the process of hybridization are:
- Selection of parents.
- Emasculation,
- Bagging,
- Tagging,
- Pollination or crossing,
- Harvesting F1 seeds,
- Further handling of the plants or distribution. Was this answer helpful?