What are Spodoptera frugiperda cell proteins?
What are Spodoptera frugiperda cell proteins?
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.
What is Sf9 insect?
The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE, and it is a suitable host for expression of recombinant proteins from baculovirus expression systems (e.g., Invitrogen’s Bac-to-Bac® and Bac-N-Blue™ Expression Systems).
Where do Sf9 cells come from?
The Sf9 cell line was cloned from the parent Sf21 cell line (IPLB-Sf-21-AE), which was derived from pupal ovarian tissue of the fall armyworm (Spodoptera frugiperda) (5).
Are Sf9 cells adherent?
Note: The cells are not really adherent, they are just resting on the plate bottom and are slightly sticky and easily dislodged.
What is the function of baculovirus?
Abstract. Baculoviruses are insect pathogenic viruses that are used for pest insect control and as tools to produce recombinant proteins. Numerous studies show that both natural baculoviruses and genetically modified (GM) baculoviruses hold clear and substantial benefits for crop protection.
What is a baculovirus vaccine?
Baculovirus is used as a vector vaccine candidate, in which the antigens can be expressed by the vector within the host cells and displayed on the baculovirus surface or plasma membrane.
Are Sf9 cells immortalized?
Of the immortalized insect cells available, the most commonly utilized lines are derived from Spodoptera frugiperda (fall armyworm) with Sf9 and Sf21 being the most common.
What is a Bacmid?
bacmid (plural bacmids) (cytology) A shuttle vector that can be propagated in both E. coli and insect cells.
How do you thaw SF9 cells?
Thawing SF9 cells Thaw frozen cells quicklyby gentle agitation in a 37°C water bath. When thawed (30-40 sec), quickly rinse the outside of the vial with 70% ethanol and proceed in the hood.
What is baculovirus expression system?
The baculovirus expression system is commonly used to generate recombinant proteins in insect cells at high production levels. Recombinant baculoviruses can accommodate large segments of foreign DNA.
What is baculovirus used for?
Baculoviruses are insect pathogenic viruses that are used for pest insect control and as tools to produce recombinant proteins. Numerous studies show that both natural baculoviruses and genetically modified (GM) baculoviruses hold clear and substantial benefits for crop protection.