Is RNAlater compatible with TRIzol?

Is RNAlater compatible with TRIzol?

Tissue stored in RNA later is compatible with all commonly used RNA isolation methods, including single reagent isolation products like Trizol®, and all of Ambion’s RNA isolation kits. It is also possible to extract both genomic DNA and total protein from samples stored in RNA later.

How do you remove the RNAlater for RNA extraction?

Blot tissues with a wipe, or pellet cells to remove excess RNAlater® Solution. Retrieve tissue from RNAlater® Solution with sterile forceps, quickly blot away excess RNAlater® Solution with an absorbent lab wipe or paper towel, and then submerge the sample in RNA isolation lysis solution.

How does RNAlater preserve RNA?

Invitrogen RNAlater Stabilization Solution is an aqueous, nontoxic tissue RNA stabilization and storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. RNAlater solution minimizes the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.

How does a RNAlater work?

RNAlater is a valuable fixative used for preservation of biological samples. The solution denatures proteases and RNases to preserve RNA and protein integrity for downstream analysis.

How do you homogenize tissue in TRIzol?

Homogenize tissue samples in 1 ml of TRIZOL Reagent per 50-100 mg of tissue using a glass-Teflon or Polytron power homogenizer. The sample volume should not exceed 10% of the volume of TRIZOL Reagent used for homogenization.

Does RNAlater lyse cells?

The cell types that have been evaluated do not lyse when frozen at –80 °C in RNAlater. Samples can subsequently be thawed at room temperature and refrozen without affecting the amount or the integrity of the recoverable RNA.

Is RNAlater a fixative?

How do you homogenize tissue for RNA extraction?

For optimal disruption of the tissue, no piece should be larger than half the diameter of the probe. Pour the minced sample into a tube containing the remaining βME/RLT buffer. Homogenize the tissue at 15-20 second intervals resting for 5 seconds between each interval for a total of 60 seconds.

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