Is pUC19 linear?

Is pUC19 linear?

pUC19 is a commonly used, high copy number cloning vector. This linearized version was generated by PCR, and contains the blunt ends shown in the MCS sequence above.

What kind of vector is pUC19?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.

What is pUC19 DNA?

Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.

Is pUC19 high copy?

What is pUC19 plasmid?

pUC19 is a commonly used plasmid cloning vector in E. The molecule is a double-stranded circular DNA (2686 base pairs in length). The pUC19 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.

What is the difference between pBR322 and pUC8?

pUC and pBR322 are both variants of the pMB1 origin. pUC has a few mutations (1 or 2 depending on who you ask) from the pMB1 sequence and these two mutations lead to a 20-35 times difference in copy number – pUC copy number is about 500 per cell while pBR322 is about 20.

What is ROP in pBR322?

ROP codes for a protein that is responsible for restricting the plasmid copy number. It is also called a repressor for primers. It is involved in the replication of the plasmid pBR322.

What is the purpose of pUC19 in the transformation?

pUC19 control DNA (10 pg/µl) is provided to check transformation efficiency. Use experimental DNA that is free of phenol, ethanol, salts, protein, and detergents to obtain maximum transformation efficiency.

Does pUC19 have lacI?

The plasmid contains an ampicillin resistance gene (Ampr), an origin of replication that functions in E. coli, and the lacI gene, which produces a repressor that blocks the transcription of lacZ’ gene in the absence of the inducer IPTG. The complete sequence of pUC19 is 2686 base pairs.

What is the function of ROP in pBR322?

Furthermore, pBR322 encodes a functional rop gene that regulates copy number. The Rop protein is involved in stabilizing the interaction between RNA I and RNA II, which in turn prevents the replication of pBR322.