Is Micrococcus luteus catalase positive?
Is Micrococcus luteus catalase positive?
Other distinguishing identification features are that M. luteus is urease & catalase positive but coagulase negative. Some Micrococcus species are now identified, particularly on newer identification techniques such as MALDI-TOF, as Kocuria sp.
Is Micrococcus luteus a catalase?
Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. It is urease and catalase positive.
Does Micrococcus produce catalase?
Micrococci are catalase-positive, oxidase-positive, strictly aerobic gram-positive cocci that grow in clusters.
Is Micrococcus luteus MSA positive or negative?
Gram-positive
Micrococcus luteus Description: ● Micrococcus luteus (M. luteus) is a Gram-positive to Gram-variable, non-motile, coccus, saprotrophic bacterium. It can form in tetrads or irregular clusters but not in chains and belongs to the family Micrococcaceae.
Does M. luteus grow on MSA?
When grown on mannitol salt agar some species of Micrococcus (Micrococcus is a normal flora of human skin, mucosa, and oropharynx), such as M. luteus (yellow) can produce yellow colonies. M. roseus (red) produces pink colonies on MSA.
Is Micrococcus luteus gamma hemolytic?
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| Genus species Gram+ | Micrococcus luteus |
|---|---|
| Morphology* | G+ cocci/packets |
| Pigment* | yes |
| Hemolysis* | gamma |
| Nitrate^ | – |
Is Micrococcus catalase positive or negative?
Micrococcus species are strictly aerobic Gram positive cocci arranged in tetrads or irregular clusters, not in chains and cells range from 0.5 to 3µm in diameter. They are seldom motile and are non-sporing. They are also catalase positive and often oxidase positive, although weakly.
What can grow on MSA plate?
Staphylococcus species
Mannitol Salt Agar (MSA) The high salt concentration (7.5%) is the selective ingredient. Staphylococcus species, which commonly inhabit human skin, can grow on this high salt concentration (left plate in picture below).
What type of hemolysis is Micrococcus?
gamma
| Genus species Gram+ | Morphology* | Hemolysis* |
|---|---|---|
| Micrococcus luteus | G+ cocci/packets | gamma |
| Mycobacterium smegmatis | G+ rod | ND |
| Staphylococcus aureus | G+ cocci/clusters | beta |
| Staphylococcus epidermidis | G + cocci | gamma |
What bacteria grows on MSA agar?
Result Interpretation on Mannitol Salt Agar
| Organisms | Results |
|---|---|
| Staphylococcus aureus | Yellow colonies with yellow zones. |
| Staphylococci other than S. aureus (e.g. Staphylococcus epidermidis ) | Colorless or Red colonies with red zones. |
| Streptococci | No growth to trace growth. |
| Micrococci | Large white to orange. |
Which bacterium is positive on MSA test?
Staphylococcus aureus
Pathogenic gram-positive Staphylococcus aureus salt-tolerant (halophilic) and will produce colonies on MSA. Staphylococcus aureus is also able to ferment mannitol, because this bacterial species has the enzyme coagulase required for the process.
What does MSA select for?
The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration (plate on the left in the picture below). This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar (plate on the right in the picture below).