How do you reticulin stains?
How do you reticulin stains?
Method
- Deparaffinise sections with xylene then take through alcohols to water.
- Oxidise in acidified potassium permanganate for 3 minutes.
- Rinse in distilled water.
- Decolourise with 2% oxalic acid for 1 min.
- Rinse in distilled water.
- Mordant in 4% iron alum for 10 minutes.
- Rinse in distilled water.
What stain is used for reticulin?
Ammoniacal silver stains
Ammoniacal silver stains are most commonly used for reticular fiber and basement membrane staining. A variety of methods are available including Snook’s, Wilder’s, Foot’s, Jones’s, and Gomori’s periodic acid-methenamine silver method (PAMS).
Which method is used to demonstrate reticulin fibers?
Gordon and Sweet’s silver staining method
The Gordon and Sweet’s silver staining method is used to demonstrate reticular (retic) fibers. This method relies on the impregnation of retic fibers with silver through oxidation and reduction. The tissue is first oxidized using potassium permanganate to enhance subsequent staining.
What are the 3 types of special stains?
Special Stains
- Connective Tissue Stains.
- Microrganisms.
- Carbohydrate Stains.
- Pigments, Minerals and Cytoplasmic Granules.
- Fat Stains.
What is a reticulin?
Reticulin is a histological term used to describe a type of fiber in connective tissue composed of type 3 collagen in which these reticular fibers crosslink to form a fine meshwork.
What is reticulin fibrosis?
Increased reticulin staining (reticulin fibrosis) is associated with many benign and malignant conditions while increased trichrome staining (collagen fibrosis) is particularly prominent in late stages of severe myeloproliferative diseases or following tumour metastasis to the bone marrow.
What stains staining techniques are used to demonstrate the reticulin fibers?
Reticulin stains use silver and rely on the argyrophilic properties of the fibers. Argyrophilic cells can adsorb silver but cannot reduce it. “Adsorb” is a surface-based process, whereas “absorb” means there is permeation through the tissue.
What is the function of reticulin?
Reticular fibers crosslink to form a fine meshwork (reticulin). This network acts as a supporting mesh in soft tissues such as liver, bone marrow, and the tissues and organs of the lymphatic system.
What is a Reticulin?
What are staining techniques?
Staining is a technique used in microscopy to enhance contrast in a microscopic image. Stains and dyes are frequently used to highlight structures in microbes for viewing, often with the aid of different microscopes.
Why do reticular Fibres appear as dark strands on a reticulin stained slide?
Periodic acid-Schiff (PAS) reaction, which takes advantage of the high polysaccharide content of reticular fibers. Gomori and Wilder silver nitrate staining procedures, which make the fibers appear black (argyrophilic).
What is reticulin in bone marrow?
Reticulin is a normal component of the bone marrow stroma and can be detected with a reticulin stain in 73% to 81% of healthy subjects. 16-19. Increased reticulin staining (reticulin fibrosis) is associated with many benign conditions as well as some malignant diseases.