Does Mycobacterium tuberculosis reduce nitrate?

Does Mycobacterium tuberculosis reduce nitrate?

Mycobacterium tuberculosis reduces nitrate very strongly as compared to Mycobacterium bovis and M. bovis BCG. Nitrate reductase, in conjunction with niacin accumulation, constitutes one of the major biochemical tests used in clinical microbiology laboratories to differentiate M.

Does M tuberculosis reduce nitrate to nitrite?

One of the activities induced in M. tuberculosis during oxygen deprivation is the respiratory reduction of nitrate to nitrite.

What does the nitrate reduction test test for?

Nitrate reduction test is a test that determines the production of an enzyme called nitrate reductase, which results in the reduction of nitrate (NO3). Bacterial species may be differentiated on the basis of their ability to reduce nitrate to nitrite or nitrogenous gases.

How do you perform a nitrate reduction test?

Procedure of Nitrate Reduction Test Add 6-8 drops of nitrite reagent A and add the 6-8 drops of nitrite reagent B. Observe for the reaction (color development) within a minute or less. If no color develops add zinc powder. Observe for at least 3 minutes for a red color to develop after addition of zinc.

How can you distinguish Mycobacterium tuberculosis and Mycobacterium bovis?

Introduction. Unlike Mycobacterium tuberculosis, which only causes human tuberculosis (TB), M. bovis is a zoonotic pathogen that infects humans and domestic animals. This infection causes bovine TB, thus posing a major economic and public health problem for the animal husbandry industry.

What indicates a positive result for nitrate reduction?

When sulfanilic acid is added, it will react with the nitrous acid to produce diazotized sulfanilic acid. This reacts with the α-naphthylamine to form a red-colored compound. Therefore, if the medium turns red after the addition of the nitrate reagents, it is considered a positive result for nitrate reduction.

Why is nitrate reduction important?

Nitrate reduction plays a key role in the nitrogen cycle and has important agricultural, environmental, and public health implications.

Is M. bovis catalase positive?

bovis and M. africanum are all negative for both the thermostable catalase test (68degC) and semi-quantitative catalase test (>45mm), M.

Is Mycobacterium bovis Gram-positive or negative?

Gram-positive
Mycobacterium bovis is a Gram-positive, aerobic, nonmotile, straight or slightly curved, rod-shaped bacterium.

What is nitrate reducing bacteria?

Nitrate-reducing bacteria are mostly heterotrophic and often facultative anaerobic, with the ability to switch between oxygen and nitrate respiration depending on the environmental conditions (Luque-Almagro et al., 2011).

Is Mycobacterium tuberculosis catalase positive or negative?

Biochemical Test of Mycobacterium tuberculosis

Is Mycobacterium catalase positive?

Mycobacteria are Gram-positive, catalase positive, non-motile, non-spore forming rod-shaped bacteria (0.2–0.6 μm wide and 1.0–10 μm long). The colony morphology of mycobacteria varies with some species growing as rough or smooth colonies.