Which medium is used for growing the hybridoma cell?
Which medium is used for growing the hybridoma cell?
The hybridoma cells are grown in Excell medium (Sigma-Aldrich) supplemented with 10 mM l-glutamine, and the cell culture supernatant is collected and stored at − 20 °C in the presence of sodium azide until needed.
What is the difference between Ti 1 and Ti 2 antigens?
TI antigens can be further divided into TI type 1 antigens (TI-1), which are polyclonal B lymphocyte activators (e.g. lipopolysaccharides), and TI type 2 (TI-2) antigens. TI-2 antigens are able to directly stimulate B lymphocytes. However, the antibody response to TI-2 antigens is somehow influenced by T lymphocytes.
Which cells are used for hybridoma?
Hybridoma is a culture of hybrid cells that results from the fusion of B cells and myeloma cells. Hybridoma technology produces hybridomas. This technology was developed to produce mAbs. Hybridomas possess two important properties of B cells, production of antibodies, and immortalization of myeloma cells.
How do you grow hybridoma cells?
To grow hybridoma cells for antibody production cultivate cells until the medium is yellow and most of the cells are died. Collect suspension in 250-500 ml centrifuge containers, spin down at +4°C for 30 min with maximum speed, transfer the supernatants to fresh tubes and repeat spinning one more time.
How are hybridoma cells produced?
Hybridomas are generated by fusing spleen cells (plasma cells) from immunized hosts with a compatible myeloma cell line (malignant and immortal plasma cell line).
What is a Ti 2 antigen?
T-Independent-2 antigen It results in proliferation and differentiation of B lymphocytes and production of antibodies. TI-2 antigens can activate only mature B lymphocytes. Immature B cells are anergized, so they do not elicit any immune response.
What is TD antigen?
TD antigens are usually soluble protein antigens that require major histocompatibility complex (MHC) class II-restricted T cell help for antibody production while TI antigens do not require such help, and are often multivalent and poorly degraded in vivo.
How do you get hybridoma cells?
In which medium do hybridoma cells grow selectively Mcq?
Complete answer: HAT(hypoxanthine–aminopterin–thymidine) medium is used for the selection of fused hybridoma cells in the process of monoclonal antibody production.
Why myeloma cells are used in monoclonal antibody production?
A typical monoclonal antibody production process Preparation of myeloma cells – Myeloma cells are immortalized cells that, once fused with spleen cells, can result in hybridoma capable of unlimited growth.