What does 2-deoxyglucose do to the cells?
What does 2-deoxyglucose do to the cells?
2-Deoxyglucose (2-DG) is a glucose analog that inhibits glycolysis9,10. 2-DG can compete with glucose to bind hexokinase (the first rate-limiting enzyme of glycolysis) in cells and inhibit metabolism of tumor cell, thereby inhibiting cell proliferation11.
Where does 2-deoxyglucose inhibit glycolysis?
Acting as a d-glucose mimic, 2-DG inhibits glycolysis due to formation and intracellular accumulation of 2-deoxy-d-glucose-6-phosphate (2-DG6P), inhibiting the function of hexokinase and glucose-6-phosphate isomerase, and inducing cell death.
Is 2-deoxyglucose a competitive inhibitor?
Although metrizamide, 2-deoxyglucose, and glucosamine are known to be competitive inhibitors of approximately equal potency for glucose of yeast hexokinase (Ki approximately 0.7 mM for all three), metrizamide is a much weaker competitive inhibitor (Ki about 20 mM) of rat brain hexokinase than either 2-deoxyglucose or …
What is the composition of 2-DG?
2-DG is up taken by the glucose transporters of the cell….2-Deoxy-D-glucose.
| Names | |
|---|---|
| Chemical formula | C6H12O5 |
| Molar mass | 164.16 g/mol |
| Melting point | 142 to 144 °C (288 to 291 °F; 415 to 417 K) |
What is the mechanism of action of 2-deoxyglucose?
The exact mechanisms of action of 2-deoxyglucose is still being investigated, but it is known that in hypoxic cancer cells, 2-deoxyglucose is a glycolysis inhibitor that prevents ATP production and, ultimately, cell survival.
What is the function of 2-DG?
2-DG is essentially a glucose molecule in which, the 2-hydroxyl group gets replaced by hydrogen. Due to this chemical replacement, 2DG is not able to enter glycolysis and contribute to ATP production.
Is D 2 Deoxygalactose the same as d2 deoxyglucose?
Is D-2-deoxygalactose the same chemical as D-2-deoxyglucose? Explain. Both cellulose and glycogen are polymers of D-glucose but they differ in their glycosidic linkage. You just studied 11 terms!
What is 2-DG used for?
1.In the 2-DG arm, 42% of the patients improved symptomatically and became free from supplemental oxygen by Day-3 in comparison to 31% in the SoC arm, indicating an early relief from oxygen therapy/dependence. 2. A higher proportion of patients treated with 2-DG showed RT-PCR negative conversion in COVID patients.
What are the side effects of 2-DG?
The effect of 2-DG is enhanced in hypoxia, which is a common phenomenon in GBs [8]. Furthermore, 2-DG induces the neuronal commitment of tumor cells [9], inhibits HIF-1α [10] as well as N-linked glycosylation [11], and sensitizes tumors cells to radiation therapy and chemotherapy [12].
How do you make 2 deoxy D glucose?
The preparation method comprises the following steps: taking 3,4,5-tri-O-acetyl D-glucal or 3,4,5-tri-hydroxy-glucal as a raw material, and is placed in a solvent under existence of solid acid, stirring and reacting for certain time under certain temperature, filtering to remove solid acid, washing the solid acid by …