What does M stand for in kinetics?
What does M stand for in kinetics?
Here they are: Km: The Michaelis constant with units of molarity (M), is operationally defined as the substrate concentration at which the initial velocity is half of Vmax. It is equal to the dissociation constant of E and S only in if E, S and ES are in rapid equilibrium.
What is meant by Michaelis-Menten kinetics?
Michaelis-Menten kinetics, a general explanation of the velocity and gross mechanism of enzyme-catalyzed reactions. First stated in 1913, it assumes the rapid reversible formation of a complex between an enzyme and its substrate (the substance upon which it acts to form a product).
What does the Michaelis-Menten equation describe?
Michaelis-Menten Equation. The Michaelis-Menten equation, which was derived previously, describes the rate of catalysis of the enzyme at some particular substrate concentration.
What is MM constant?
The Michaelis constant Km is equal to the reactant concentration at which rA=vmax/2. Km is independent of enzyme concentration but varies from one enzyme to another and with different substrates for the same enzyme.
What does Vmax mean?
Vmax is the reaction rate when the enzyme is fully saturated by substrate, indicating that all the binding sites are being constantly reoccupied. From: Introduction to Biological and Small Molecule Drug Research and Development, 2013.
What is Michaelis-Menten constant significance?
Significance of Michaelis-Menten Constant: (i) By knowing the Km value of a particular enzyme-substrate system, one can predict whether the cell needs more enzymes or more substrate to speed up the enzymatic reaction.
What is the definition of Vmax?
The maximal velocity of the reaction (or maximal rate) Vmax is the rate attained when the enzyme sites are saturated with substrate, i.e. when the substrate concentration is much higher than the KM. Examples: Q8W1X2, Q9V2Z6.
What is Vmax in enzyme kinetics?
The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, Vmax.
What is a Vmax in enzyme kinetics?