What are C2C12 myoblast cells where did they come from?
What are C2C12 myoblast cells where did they come from?
C2C12 cells were originally derived from satellite cells from the thigh muscle of a 2-month-old female C3H mouse donor 70 h after a crush injury. They readily proliferate in high-serum conditions, and differentiate and fuse in low-serum conditions.
What are C2C12 myotubes?
C2C12 myotubes express contractile proteins and, when left to differentiate for an extended period of time, can spontaneously contract. These properties make C2C12 cells an invaluable tool for understanding the molecular biology of muscle development. However, these cells do not perfectly mimic in vivo muscle fibers.
What type of cell is myoblast?
2.1 Skeletal Myoblasts Skeletal myoblasts are the first cell types to be studied for the treatment of cardiomyopathy and heart failure. Skeletal myoblasts are progenitor cells, located between the basal lamina and sarcolemma, and are activated in response to muscle damage or disease-induced muscle degeneration.
Are C2C12 cells immortal?
In fact, C2C12 cells are immortal, proliferate rapidly, and will not differentiate unless induced.
What are C2C12 cells?
C2C12 is a myoblast cell line that is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.
What type of cell is C2C12?
mouse myoblast cell line
C2C12 is an immortalized mouse myoblast cell line. The C2C12 cell line is a subclone of myoblasts that were originally obtained by Yaffe and Saxel at the Weizmann Institute of Science in Israel in 1977.
What is C2C12 cell line?
What is myoblast cell?
A myoblast is an embryonic precursor cell that differentiates to give rise to the different muscle cell types. Differentiation is regulated by myogenic regulatory factors, including MyoD, Myf5, myogenin, and MRF4. GATA4 and GATA6 also play a role in myocyte differentiation.
What is a C2C12 cell?
What is a myoblast?
Definition of myoblast : an undifferentiated cell capable of giving rise to muscle cells.
How do you differentiate C2C12 cells?
For differentiation, we switch to 5% horse serum, 1X penicillin-streptomycin in DMEM while C2C12 myoblasts is about 60-80% confluent. I prefer change fresh medium everyday for 5-7 days to get the best result (occasionally 2 days will be OK).