What is microalgal biomass?
What is microalgal biomass?
Microalgal biomass is made up of cells with a significant protein content, even after a carbon-accumulating secondary cultivation phase.
How is algal biomass measured?
Simply compare the biomass on successive time points, usually days. The specific growth rate (μ) of the microalgae is calculated using the equation μ = ln(N2/N1)/(t2 − t1), where μ is the specific growth rate, and N1 and N2 are the biomass at time 1 (t1) and time 2 (t2), respectively. This is a unitless measurement.
What is algal biomass used for?
Algal biomass is regarded as a promising substrate for biofuel generation. There are many kinds of biofuels, which include biodiesel, bioethanol, biohydrogen, and biomethane. Depending on the type of biofuel intended to synthesize, different processes of biofuel synthesis from algal biomass are employed (Fig. 17.3).
How is algae growth measured?
Gravimetric measurement of ash-free (organic) dry weight (AFDW) is by far the most reliable method for measuring growth and also calculating the productivity of algae cultures and is the best for comparing different algae. The ash content of dry microalgae is usually at least 8–10 % of the dry weight.
What is microalgal biotechnology?
Microalgae are a diverse group of photosynthetic microorganisms that produce several useful products including biodiesel feedstock, bioactive molecules, human food, and animal feed. They can also be used in biological carbon dioxide sequestration.
How is biomass productivity calculated?
Biomass Growth Analysis Growth rate was calculated by the formula, μ = Ln (N2/N1)/(t2–t1), and biomass productivity was calculated by the formula, biomass productivity (g L−1 d−1) = (N2–N1)/(t2–t1), where N1 and N2 are defined as the biomass at time 1 (t1) and time 2 (t2), respectively.
How do you determine biomass?
Total biomass is found by summing the dry mass biomass of all individuals in a given land area and then reported by naming the area of concern, e.g. biomass per plot, ecosystem, biome, classroom. To be able to compare biomass in different locations, scientists standardize biomass per unit of area.
How is algal density measured?
Density (cells/mL) could be estimated as follow: Cell Density = e {[ln (absorbance_684) +16.439]/1.0219} (n=130; r2=0.9998). Residual distribution revealed that the equation could be applied for algal densities under 5,000,000 cells/mL and/or absorbance values as high as 0.5.
How is algae extracted from biomass?
The Algal oil was separated from Algae biomass by filtration and weighted it by using electronic weight balance. The extracted oil was evaporated in a water bath to release hexane. All extraction was performed in triplicates for the different parameters solvent extraction process.
How does a spectrophotometer measure algae growth?
The OD should measure 670 nano meters (nm). Turn the algal cell upside down and shake it gently for 10 seconds. Check the algae sample’s optical density by viewing the algal cell beneath the spectrophotometer and measuring the algal cell’s OD.
How do you measure algae concentration?
A very simple method is to allow the sample to settle in a graduated cylinder and measure the volume. This is faster if the cells are killed with alcohol, formalin or an iodine solution called Lugol’s iodine all are toxic to humans. Samples can be concentrated using a centrifuge, if that is available.
What is the difference between microalgae and macroalgae?
What is the Difference Between Macroalgae and Microalgae? Macroalgae are large aquatic photosynthetic plant-like organisms that are visible to our naked eye. They are commonly referred to as seaweeds. Meanwhile, microalgae are small aquatic photosynthetic plant-like organisms that are visible only under the microscope.